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KMID : 0525720150200010051
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Journal of Chitin and Chitosan
2015 Volume.20 No. 1 p.51 ~ p.56
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Screening of Cellulolytic Microorganism and Characterization of Cellulase from Bacillus licheniformis CEL-2
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Kwon Eun-Ryeong
Seo Jung-Min
Lee Ok-Jin
Kim Jun-Ho
Jung Young-Ho
Kim Yeong-Su
No Hong-Kyoon
Park Chang-Su
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Abstract
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We carried out screening of microorganisms producing cellulase from Korean traditional fermented foods. As a result, we isolated 5 strains as cellulolytic microorganisms due to their ability of forming active zone on LB-Agar plate containing carboxymethylcellulose (CM-cellulose) as substrate with trypan blue. Among one of the strains, one strain was selected because its culture supernatant showed the highest cellulase activity for CM-cullulose. In the analysis of 16S rRNA gene sequence from the strain, the isolated strain showed 99% of identity with Bacillus licheniformis, therefore we named the strain Bacillus licheniformis CEL-II. The optimum pH and temperature of cellulase from B. licheniformis CEL-II were the highest activities at pH 5.0 and 60 o C, respectively. In the investigation of cellulase activity for several substrates such as CM-cellulose, alkali swollen cellulose, α-cellulose, Sigma Cellulose and Avicel, the cellulase from B. licheniformis CEL-II showed the highest activity for CM-cellulose. From the analysis of enzyme reaction products obtained from the hydrolysis of alkali swollen cellulose, the cellulase from B. licheniformis CEL-II was characterized to be an endo-type enzyme because the various size cellooligosaccharides were detected as main products on the analysis by thin-layer chromatography.
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KEYWORD
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Fermented Food, Screening, Bacillus licheniformis, Cellulase, Characterization
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